Untrained users must contact core personnel to schedule handson training. It is set up to detect up to 10 fluorescent parameters simultaneously. The hts will initialize automatically, otherwise go to hts. The bd lsriis and bd lsrfortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. Its capability of detecting up to 16 parameters and 14 colors simultaneously makes it ideal for multicolor flow cytometry assays that involve a large panel of markers. Bd biosciences digital flow cytometry software provides. The bd lsrii is a tencolor benchtop flow cytometer. Preconfigured bd lsr ii based on over 10 years of experience in the support of core laboratories and advanced research requirements, bd provides a selection of preconfigured bd lsr ii analyzers recommended for multicolor flow cytometry research. Approximately half the size of the bd lsr ii, it can easily fit on the benchtop for cost effective space utilization. Bd lsr fortessa flow cytometry analyzer equipped with hts high throughput sampler the facility at 2117a everitt lab click link for campus map is equipped with a bd lsr fortessa analyser. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications. Of the six benchtop analyzer flow cytometers in the lab, four are manufactured by bd biosciences, they are a 2laser facscanto running facsdiva 8 software, a 2laser facscalibur running cellquest pro software and two lsr ii sorp instruments one 4laser and one 5laser running facsdiva 8 software. Sevenlaser excitation of seven fluorochromes is shown, demonstrating the utility of a sevenlaser special order bd lsr ii analyzer. Lrb lsr a 4b 2v 2r 2uv uncle fester has 4 lasers and 10 fluorescent detectors and lrblsr b 2b 3v 3r 5yg lurch has 4 lasers and fluorescent detectors.
The bd lsrfortessa x20 cell analyzer delivers highperformance multicolor analysis with the most compact footprint in its class at just 30 x 29 76. The bd lsrfortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Gladstone flow cytometry core miltenyi macsquant vyb. Bd bioscience facscanto ii 3 laser flow cytometer used. This webinar covers the basic components of a flow cytometer, how to interpret a dye excitationemission spectrum, how data is displayed, basic gating. Recently upgraded, now with four lasers, for 12color analysis. It has five lasers with excitation lines at 355nm, 407nm, 488nm, 561nm, and 640nm. There are 2 lsr iis housed in the lazare research building. Bd lsrii overview workbook center for cancer research. The bd lsr ii flow cytometer is a flexible and powerful bench top analyzer available to uta researchers. This system is dedicated for analytical use, and is able to detect up to 16 colors per cell simultaneously, allowing for highly complex cocktails of antibodies for.
Innovative technology in the bd lsr ii optics and digital electronics have created a more sensitive. Bd biosciences launches new cell analyzer for complex. Software operating system microsoft windows 7 bd facsdiva software version 8. Bd lsr ii flow cytometer with the ability to detect up to 10 colors per sample.
It can collect up to 14 fluorescent parameters and two light scatter parmeters. The bd lsriis and bd lsrfortessa can be configured with up to five fixed alignment. The rockefeller university bd lsriis and bd lsrfortessa. Used bd biosciences lsrii flow cytometer 2032176 flow. The bd facscanto system breaks new frontiers in flow cytometry by providing an efficient, easytouse yet powerful sixcolor benchtop analyzer. Bringing boundless potential to multicolor flow cytometry, the bd lsrfortessa x20 cell analyzer gives you the power to expand and customize your science.
In addition, the advanced flow system allows high speed sample runs. Free up your highperformance cell sorter by moving nonsorting applications that require a uv or krypton laser to the bd lsr ii. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by bd expert training, service and supportso there is no limit to your research. Bd biosciences lsr ii flow cytometer marshall scientific. The bd lsrfortessa system offers power and flexibility in a benchtop footprint that is significantly smaller than the industry leading bd tm lsr ii cell analyzer. The bd lsr ii flow cytometer is equipped with four lasers 355, 405, 488 and 633 nm in addition to a sophisticated bd high throughput sampler that allow rapid collection of samples directly from a 96well plates and the latest bd facsdiva software. Fourlaser, 18color benchtop flow cytometer building on the ease of use and flexibility of the bdtm lsr, the first uv benchtop analyzer, bd biosciences introduces the bdtm lsr ii. Bd facsdiva software drives experiment design, instrument control, and data analysis on the bd lsr ii system. Rightclick a specimen or experiment and select batch analysis using a global worksheet.
Bd lsr ii digital flow cytometry analyzer this instrument is one of the most powerful and flexible flow cytometry analyzer on the market. Bd lsrfortessa optical configuration bd lsrii1 default filter configuration 16. Acquisition, analysis, and most bd lsr ii cytometer functions are controlled by. Bd facscanto ii cell analyzer with proven reliability and highquality results supporting up to 10 parameters. It is the only compact cell analyzer that supports up to five lasers. Lasers blue laser, 488nm red laser, 633nm detectors forward scatter detector is a photodiode five wave lengths and side scatter are detected from the 488nm laser 53030 nm fitc, alexa fluor 488, gfp, cfse, calcein, yfp, r123, mito green, syto 1418, yopro3, fluo4 57526 nm pe 61020 nm petexasred, propidium iodide, pealexa flour 610 69540 nm pecy5. Bd lsrii digital flow cytometry analyzer this instrument is one of the most powerful and flexible flow cytometry analyzer on the market. Biocenter flow cytometry unit is located at the viikki campus and provides flow cytometry analysis services and training for new users. The special order bd lsrfortessa cell analyzer puts the power of the proven bd lsr platform into a compact footprint.
The lsr ii from becton dickinson bd is an advanced analyzer equipped with 4 lasers emitting at 488 nm blue, 633 nm red, 405 nm violet, and 355 nm ultraviolet. The bd lsr ii flow cytometer is the most flexible yet powerful benchtop analyzer available. The cytometer allows detection of 12 parameters simultaneously 10 colors plus forward scatter and side scatter. This instrument is used for many applications including fret measurements. It uses fixedalignment lasers that transmit light reflected by mirrors through a flow cell to octagon and trigon detector arrays. The bd lsr ii is a flow cytometer equipped with 4 lasers.
The bd lsrfortessa puts the power of the bd lsr platform into a compact footprint, about half the size of the bd lsr ii, and. Training for using the machines is provided by the manager of. For each tube collected in diva software, uncompensated data is recorded and the compensation matrix is applied as a transform, it does not affect your data. This video describes how to set up an experiment in facs diva version 8. Flow cytometry technologies flow cytometry shared resource. The lsr ii is a digital multiparameter flow cytometer analyzer.
Facs cantoii analyzer threelaser, fully digital facs cantoii analyzer, used for up to eightcolor tenparameter analysis of cell samples. Innovative technology in the bd lsr ii optics and digital electronics have created a more sensitive flow cytometer that yields more information from each sample. The bd facscalibur platform allows users to perform both cell analysis and cell sorting in a single benchtop system. Running a basic 2 color flow cytometry experiment in bd.
It is operated by users after basic training in operation, maintenance, and troubleshooting. Lsrii analyzer sophisticated, analytical instrument, used for complex analysis of cell samples. Gladstone flow cytometry core intellicyt high throughput flow cytometer. The bd lsrfortessa puts the power of the bd lsr platform into a compact footprint, about half the size of the bd lsr ii, and is designed to enable your current assay requirements. Innovative technology in the bd lsr ii optics and digital electronics have created a. Cell analysis u manitoba flow cytometry core facility. Bd facsdiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the bd lsrfortessa workstation. Lsr ii analyzer sophisticated, analytical instrument, used for complex analysis of cell samples. The instrument is available for trained users in a selfoperated mode.
Note that these choices are based upon bd instruments bd facsverse, bd lsr cell analyzer platform, bd facsaria cell sorter platform, or bd facs canto. The lsr ii has four lasers, 405, 488, 552, and 637 nm, and can detect light in seventeen photomultiplier tubes pmts and one photodiode. Flow cytometer analyzers and sorters research resource program. Bd lsr ii flow cytometer system research and innovation. The bd lsr ii utilizes bd facsdiva software, which provides a means for experiment design, instrument control, and data analysis. Building on the ease of use and flexibility of the bdtm lsr, the first uv benchtop analyzer, bd biosciences introduces the bdtm lsr ii. Up to eight different fluorescent probes can be measured simultaneously. The bd lsr ii is equipped with three lasers 405, 488 and 635 nm and can handle advanced multicolor applications.
Cell sorters special order products bd biosciencesus. Bd biosciences reagents fastimmunue cytokine detection. Bd lsr ii system from bd biosciences selectscience. Clinical software research instruments research cell analyzers. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for. It incorporates many technology advances first developed in the bd lsr ii expandable analyzer and the bd facsaria cell sorter. Flow cytometers that identify, count, and characterize cells to support cell analysis. The lsr ii bd biosciences is a fourlaser, sixteencolor cell analyzer. Service for bd lsr ii systems and their related options will continue until september 30, 2022. Bd lsriis and bd lsrfortessa have performance, capabilities, and flexibility never seen before in a benchtop analyzer. Flow cytometer analyzers and sorters research resource. Bd lsrfortessa users guide facsdiva software reference manual.
Hts option for the bd lsrfortessa cell analyzer provides rapid, fully automated sample acquisition from microtiter plates. Becton dickinson lsr ii the bd lsr ii is an aircooled 4laser bench top flow cyto meter with the ability to acquire parameters for a color experiment. Lsrii is a 4laser, 18color instrument compatible with the 96well plate sampler. Updated software terminology and screen shots for bd facsdiva software. The lsr ii is driven by bd facsdiva software with features such as. Visualsonics vevo 770 ctac zeiss axioplan 2 ctac u f icbr your pursuit is what drives us. Designed for limited space and boundless potential. The software is common across bd facs instrument families, including the bd facscanto cell analyzer and bd facsaria cell sorter systems. The lsr ii offers high acquisition speeds, full inter and intralaser compensation matrix, and multiple thresholds on all lasers. The bd lsr ii is the first and only aircooled, fourlaser benchtop flow. The bd lsr ii is the first and only aircooled, fourlaser benchtop flow cytometer with the ability to acquire up to 18 colors. Instruments support research cell analyzers bd lsr ii. Dec 28, 2016 this webinar covers the basic components of a flow cytometer, how to interpret a dye excitationemission spectrum, how data is displayed, basic gating demonstration, and common statistics and. Bd facsdiva software quick reference guide for the bd lsr ii.
It is very similar in operation to the lsr ii cytometers ral and erml. The bd lsrfortessa system offers power and flexibility in a benchtop footprint that is significantly smaller than the industry leading bdtm lsr ii cell analyzer. Bd facscanto ii cell analyzer with proven reliability and highquality. We offer stateofart analyzing flow cytometers for customer use. The lsrii offers high acquisition speeds, full inter and intralaser compensation matrix, and multiple thresholds on all lasers. To determine which fluorophores may be run on each instrument, consult this document analyzers sorters. The bd lsrii is a flow cytometer equipped with 4 lasers. It is very similar in operation to the lsr ii cytometers ral and erml satelite facilities but with greater analysing sensitivity.
Flow cytometry is a powerful technique for simultaneously analyzing. Running a basic 2 color flow cytometry experiment in bd facs diva. Available instruments stanford shared facs facility. Each spatially separated beam provides discrete fluorochrome excitation so that virtually no computer compensation is required for complete cell population separation. At the heart of the bd lsr ii is the new digital acquisition, allowing for additional parameters to be detected, and yielding more information per cell. It features the digital diva hardware and software. The bd lsr ii has performance, capabilities, and flexibility never seen before in a benchtop analyzer.
Contact us general information, help and analyzer training. Running a basic 2 color flow cytometry experiment in bd facs. Hours 247 for those who have been trained by our staff. Free up your highperformance cell sorter by moving your applications that require a uv laser to the bd. Aug 17, 2017 this video describes how to set up an experiment in facs diva version 8. An affordable and reliable analyzer, the bd lsr ii is. Bd biosciences digital flow cytometry software provides both online and offline. Its current laserdetector configuration provides 8violet, 2blue, 5green, and 3red laser excited detection channels. Bd accuri c6 plus new generation accuri makes flow cytometry even more within reach.
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